Characterization of bacteriophages with specific for antibiotic-resistant <em>Salmonella </em>Typhimurium — ASN Events
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Characterization of bacteriophages with specific for antibiotic-resistant Salmonella Typhimurium (#231)

Maheswaran Easwaran , Juhee Ahn

Bacteriophage has received renewed attention as an alternative treatment for antibiotic-resistant bacterial infections. This study aimed to investigate the possibility of using bacteriophages in controlling antibiotic-resistant Salmonella Typhimurium. The bacteriophages were characterized by phage adsorption, lytic activity, and specificity against S. Typhimurium KCCM 40253 (STKCCM), S. Typhimurium ATCC 19585 (STWT), ciprofloxacin-induced antibiotic-resistant S. Typhimurium ATCC 19585 (STCIP), and S. Typhimurium CCARM 8009 (STCCARM). The host ranges of phages, P22 and PBST-35, were narrow and specific to STKCCM. The highest rates of P22 adsorption were 78%, 76%, and 90%, respectively, against STKCCM, STWT, and STCIP, while the lowest adsorption rate was observed in STCCARM (73%). The latent period of P22 was 20 min against STWT and STCIP, having burst sizes of 250 and 514 PFU/cell. Respectively, at MOI of 1. The highest lytic activity of P22 was observed against STKCCM, STWT, and STCIP at 12 h post-infection. No noticeable lytic activity was observed against STCCARM. The results would provide useful information for better understanding the phage specificity for antibiotic-resistant pathogens.

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