Uropathogen Detection Comparison of Enhanced Quantitative Urine Culture Protocol and Standard Urine Culture Protocol in Patients with Urinary Tract Infection: Evidence Based Case Report — ASN Events
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  2. Poster Session II
  3. Uropathogen Detection Comparison of Enhanced Quantitative Urine Culture Protocol and Standard Urine Culture Protocol in Patients with Urinary Tract Infection: Evidence Based Case Report

Uropathogen Detection Comparison of Enhanced Quantitative Urine Culture Protocol and Standard Urine Culture Protocol in Patients with Urinary Tract Infection: Evidence Based Case Report (#369)

Samuel Dominggus Chandra Siahaan 1 , Ginova Nainggolan 2
  1. Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, DKI Jakarta, Indonesia
  2. Renal and Hypertension Division of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, DKI Jakarta, Indonesia

Background: Good management of urinary tract infection is strongly supported by the detection of uropathogen in patients’ urine. Untill now, the standard protocol of urine culture is known as the gold standard for uropathogen detection. Currently, a new protocol known as the Enhanced Quantitative Urine Culture (EQUC) protocol is considered capable of optimizing uropathogen detection in patients with urinary tract infections.

Objective: To find out whether the EQUC protocol can optimize uropathogen detection compared to the standard urine culture protocol.

Method: Literature search was done with the help of boolean operator in several medical journals such as Pubmed, Proquest, Cochrane, and Clinicalkey. Literature found from the search was appraised using validity, importance, and applicability (VIA) method according to the Oxford Centre of Evidence-Based Medicine critical appraisal worksheet.

Result: The literature search had found one appropriate literature suitable for the critical appraisal. After critical analysis, EQUC protocol has a sensitivity of 63.7%, specificity of 84.1%, positive predictive value (PPV) of 90.9%, and negative predictive value (NPV) of 48.2% compared to the standard urine culture protocol. The EQUC protocol is also capable of detecting 110 variations of uropathogen from 75 urine samples, while standard protocol is only capable of detecting 55 (50%) variations of uropathogen.

Conclusions: EQUC is considered to be better in term of variability for uropathogen detection compared to the standard urine culture protocol and good enough to replace standard urine culture protocol in term of diagnostic value, but is considered not to replace the standard urine culture protocol in terms of cost and time required.

  1. Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ. Urinary tract infections: epidemiology, mechanisms of action and treatment options. Nat Rev Microbiol. 2015 May; 13(5):269-84.
  2. Kasper DL, Fauci AS, Hauser SL, Longo DL, Jameson JL, Loscalzo J. Harrison’s principles of internal medicine. 19th ed. New York:McGraw-Hill; 2015.
  3. Colgan R, Nicolle LE, McGlone A, Hooton TM. Asymptomatic bacteria in adults. Am Fam Physician. 2016 Sep; 74(6):985-90.
  4. Price TK, et al. The clinical urine culture: enhanced techniques improve detection of clinically relevant microorganisms. J Clin Microbiol. 2016 May; 54(5):1216-22.
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