Pleurisy has a huge impact in the Australian pig industry at both the production stage and the abattoir. In recent years, an increase in pleurisy at the abattoir has been noted in Australia and overseas. Yet there is limited knowledge of the bacteria and viruses involved in pleurisy in Australia. Hence this study investigated the bacterial agents and porcine circovirus-2 found in lungs and lymph nodes, respectively, in slaughter pigs with pleurisy from a South-East Queensland abattoir. Forty-six farms were sampled with five lungs from pigs with pleurisy sampled from each farm. The lungs were scored, and the presence of key pathogens in the lungs determined. Lungs were sampled, cultured onto BA/SN plates and the growth being scored. The most prevalent bacteria were Mycoplasma hyopneumoniae and Streptococcus suis, found from 34 and 38 farms, respectively. Thirty-one farms had positive results for PCV2. Other bacteria were Actinobacillus species (29 farms), Pasteurella multocida (24 farms), Mycoplasma flocculare (9 farms), Actinobacillus pleuropneumoniae (7 farms), Mycoplasma hyorhinis (4 farms), Haemophilus parasuis (1 farm), Bisgaaard Taxon 10 (1 farm), Streptococcus minor (1 farm) and Streptococcus porcinus (1 farm). Most of the farms had more than one species of bacteria detected. Most of the bacteria were in low numbers, however, some species such as S. suis, P. multocida and A. pleuropneumoniae, were in high numbers. These species are associated with chronic respiratory disease and are common in pigs with pleurisy at the abattoirs in other countries.